DNA

Part:BBa_K2005011:Design

Designed by: Jarrod Shilts   Group: iGEM16_Vanderbilt   (2016-10-12)


Dipyrimidine-35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To generate this artificial DNA sequence, we wrote an algorithm that modified a seed sequence until it met parameters for the number of dipyrimidine sites and other constraints to make it compatible with current DNA synthesis restrictions . In addition, the sequence was designed to include sites for annealing by ActB qPCR primers to facilitate sequence-specific amplification and further analyses.


Source

Synthetic DNA

References